Composite

Part:BBa_J22076:Design

Designed by: Sugat Dabholkar   Group: iGEM06_Bangalore   (2006-09-04)


R0011 R0062


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 61


Design Notes

This composite part is a combination of promoters in lac regulation and lux regulation, designated to enable its downstream expression be controlled by the addition of exocellular IPTG. The pLac(Lac promoter, BBa_R0011) will be: ·negatively regulated by LacI, the Lac inhibitor (BBa_C0012). ·induced by IPTG. The lux pR(lux promoter, BBa_R0062) will be: ·activated by LuxR activator protein combined with autoinducer molecule HSL(homoserine lactone) Hence, this composite part, as the recieving part of lux self-regulating system, will only be activated when exocellular IPTG is added to activate the Lac promoter. And the amount of IPTG added can determine the expression by lux system.

Source

from registry

References